Seismic lines are 1.5 to 10 m wide linear corridors associated with petroleum exploration that fragment the landscape of northern Alberta. After clearing, seismic lines frequently exhibit low seedling recruitment and growth rates, due in part to the altered hydrologic conditions on the line. This research quantifies how seismic lines alter soil hydrophysical properties, hydrologic properties and snow accumulation patterns at two sites south of Fort McMurray, Alberta. During 2021 and 2022, water table levels were measured weekly during the summer months and were paired with measurements of soil properties (bulk density, saturated hydraulic conductivity, porosity and specific yield) on seismic lines and in the adjacent matrix. Bulk density increased on seismic lines by 19% and 113% in lowland and upland ecosystems, respectively. Saturated hydraulic conductivity decreased on seismic lines by one order of magnitude for both upland and lowland ecosystems. Water table variability was increased on seismic lines, but depth to water table was related to external factors such as topographic position. Snow measurements in March 2022 indicated that snow water equivalent was greater on seismic lines that were oriented in the East–West (EW) direction and on lines that were widest compared to SWE within the adjacent matrix. Our results provide information to researchers and industry on the alteration of important ecosystem factors on seismic lines that may limit line recovery trajectories. This information will help to guide restoration planning decisions through an enhanced knowledge of limiting factors for vegetation growth on seismic lines.